“如果没有96道移液器我们该怎么办?”

2019-07-19 10:14:48

UNT的应用生理学实验室专注于两个关键领域的研究,即体重变化的生理学和免疫学后果,以及使用营养对策最大限度地提升运动后的免疫系统健康,另外他们也为相关领域的其他研究小组进行分析和测试。Brian McFarlin教授解释说:“我们要对蛋白质和RNA生物标志物进行大量的多重分析,这需要精确的移液操作。以其中一项研究为例,我们要为每个血液样本进行50次流式细胞术制备,总样本量超过2,000个。这项工作仅由一名研究生和我来完成。很快,我们决定寻找一种解决方案来减少所需的手动移液工作量!”

Brian继续说道:“我们寻遍市场,但在售的解决方案都是固定容量系统,这意味着我们需要多达五个独立平台。我在生物标志物会议上偶然发现了当时还未上市的VIAFLO 96,在看到它能够通过更换模块获得不同量程的能力后,我意识到这正是我所需要的。会议现场的演示样机被打包寄送给我进行试用,从那时起就一直在使用!”

Brian McFarlin works with INTEGRA VIAFLO 96 channel pipette

VIAFLO 96提供的电动解决方案与传统手持式移液器一样易于使用,具有更高的可重复性和通量。 “它是我们工作上绝对的救星,我们用它进行储存管到试剂板的样品转移以及试剂添加,特别是在Luminex®分析和ELISA过程中。将试样从手持移液器转移到VIAFLO 96上是无缝的,INTEGRA会帮助我们编写针对特定应用的流程,并协助构建这些程序。支持团队向我们演示了如何使用VIALINK移液器管理软件。它非常直观,现在我们已经可以轻松地调节流程中各个环节的体积。在此之后我们还添置了非常适合孔板转移工作的VIAFLO电动移液器和VOYAGER可变枪头间距移液器。我们还有一台ASSIST,用于部分标准曲线制备的自动化,目前也有计划为我们的工作流程购置一台ASSIST PLUS。”

“我们现在只用INTEGRA移液器进行所有液体转移工作,因为它们不仅能够极大地提高我们的通量,节省大量时间,而且让我们的检测方法性能也有所提升。我们的结果具有更高的可重复性,同时出现错误的风险得以降低——它彻底改变了我们的工作流程”,Brian总结道。


The Applied Physiology Laboratory at UNT focuses on research in two key areas – physiologic and immunologic consequences of weight change, and using nutritional countermeasures to maximize immune health after exercise – and performs analysis and testing for other research groups in related fields. Professor Brian McFarlin explained: “We conduct a lot of multiplexed analysis of protein and RNA biomarkers, and this requires precise pipetting. For one study, we were performing 50 flow cytometry preps per blood sample, and we had over 2,000 samples. The work was only between one graduate student and myself, and we quickly decided that we needed a solution to reduce the amount of manual pipetting we were carrying out!”

Brian continued: “We looked around the market, but the solutions on offer were fixed volume systems, meaning we would need up to five separate platforms. I first learned about the VIAFLO 96 multichannel pipette at a biomarker conference before it was even available and, after seeing its ability to change modules to have different volumes, I knew it was just what I needed. The conference’s demo model was packed up and sent to me for a trial, and it’s been in constant use ever since!”

Brian McFarlin works with INTEGRA VIAFLO 96 channel pipette

The VIAFLO 96 offers an electronic solution that is as easy to use as a traditional handheld pipette, with improved reproducibility and throughput. “It’s been an absolute lifesaver for our work; we use it for sample transfer from storage tubes to reagent plates and reagent additions, especially for running Luminex® assays and ELISAs. Transferring the assays from handheld pipettes on to the VIAFLO 96 was seamless; INTEGRA was able to support us by writing protocols for specific applications and assisting in the set-up of these programs. The team showed us how to use the VIALINK pipette management software which is very intuitive, and now it’s easy for us to tweak a volume here or there on the protocols. We have since added VIAFLO electronic pipettes and VOYAGER variable tip spacing pipettes – which are great for our plate transfer work – to our collection. We also have an ASSIST that we use to automate some of our standard curve development, and we’re now looking to purchase an ASSIST PLUS pipetting robot for our workflow as well.”

“We now exclusively use INTEGRA pipettes for all of our liquid transfer work because of the benefits they offer; not only has our throughput increased tremendously, saving a lot of time, but the performance of our assays has improved. Our results are much more reproducible and there is a reduced risk of error – it’s revolutionized our workflow,” Brian concluded.  


电话咨询
邮件咨询
在线地图
QQ客服